The c-Myc expression and apoptosis of mouse thymocytes treated with dexamethasone
- VernacularTitle:地塞米松影响小鼠胸腺细胞c-Myc表达以及凋亡研究
- Author:
Tong WANG
;
Yaoying ZENG
;
Xiaokun LI
;
Jingxian ZHAO
;
Qing WANG
;
Xiaobing FU
- Publication Type:Journal Article
- Keywords:
Dexamethasone;
Thymocytes;
Apoptosis;
c-Myc expression;
Caspase-3
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study c-Myc expression and its relationship with caspase-3 in a dexamethasone (DEX)- induced mouse thymocyte apoptosis model, and discuss the role of c-Myc in cell apoptosis. METHODS: Mouse thymocyte apoptosis was induced by 1 ?mol/L DEX, the apoptotic and necrosis cells were measured by Annexin V-FITC/PI double staining flowcytometry at 30 min, 3 h, 6 h and 9 h . Electron microscopy observation was carried out at 6 h, and c-Myc and caspase-3 contents were tested by Western blot at 0, 30, 60, 180 min. RESULTS: By 1?mol/L DEX treatment, the apoptosis rates of thymocytes at 30 min, 3 h, 6 h, 9 h were (5.70?0.46)%, (35.79?1.13)%, (50.61?2.15)% and (35.52?1.66)%,respectively; in control group, they were (5.97?0.25)%, (10.20?0.71)%, (12.10?0.66)% and (15.45?0.51)% (P
