Effects of glycine on intracellular free calcium and tumor necrosis factor-? in cardiomyocytes during hypoxia/reoxygenation
- VernacularTitle:甘氨酸对缺氧/复氧心肌细胞[Ca~(2+)]i和TNF-?浓度的影响
- Author:
Xiaojuan LI
;
Daxiang LU
;
Huadong WANG
;
Renbin QI
;
Yanping WANG
;
Chujie LI
- Publication Type:Journal Article
- Keywords:
Anoxia;
Glycine;
Tumor necrosis factor
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the influence of glycine on intracellular free calcium, the concentration of tumor necrosis factor-? and the survival rate of myocardial cells during hypoxia/reoxygenation (H/R). METHODS: The simulated model of myocardial ischemia-reperfusion with the primary cultured cardiomyocytes of neonatal rats was established, and the cultured cardiomyocytes were divided into seven groups, control group, hypoxia/reoxygenation group, glycine (0.5 mmol/L) plus hypoxia/reoxygenation group, glycine (1.0 mmol/L) plus hypoxia/reoxygenation group, glycine (2.0 mmol/L) plus hypoxia/reoxygenation group, glycine (4.0 mmol/L) plus hypoxia/reoxygenation group, 4.0 mmol/L glycine group. RESULTS: Within certain concentration (0.5-2.0 mmol/L), the glycine could inhibit the calcium overload resulting from hypoxia/reoxygenation injury in cells in a dose-dependent manner with the optimal inhibitory effect at 2.0 mmol/L. Glycine inhibited the secretion of tumor necrosis factor-? from myocardial cells and increased the survival rate of myocardial cells. CONCLUSION: Glycine has a protective effect on hypoxia/reoxygenation myocardial cells, which may be related to inhibiting calcium overload and decreasing the production of tumor necrosis factor-?.
