The regulation of peroxisome proliferator-activated receptor-? on apoptotic related genes p53 and bcl-2 in cultured vascular smooth muscle cells
- VernacularTitle:过氧化物酶体增生物激活物受体?诱导的平滑肌细胞凋亡过程中相关基因p53和bcl-2表达的变化
- Author:
Zhaohui WANG
;
Qiong GAN
;
Shaojie HAN
;
Yuhua LIAO
- Publication Type:Journal Article
- Keywords:
Receptors, peroxisome proliferator-activated;
Muscle, smooth;
Apoptosis;
Genes, p53;
genes, bcl-2.
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: We sought to evaluate the expression of apoptosis related genes, p53 and bcl-2, in cultured vascular smooth muscle cells (VSMCs) in which apoptosis was induced by the ligands of PPAR?-ox-LDL,ciglitazone and 15-deoxy-△ 12,14-PGJ 2,and then influenced by PGF 2?,an antagonist of PPAR?. To clarify whether PPAR? is involved in the regulations of p53 and bcl-2.METHODS: Rat aorta smooth muscle cells were isolated and cultured in DMEM containing 10%FBS. ox-LDL,ciglitazone and 15 d-PGJ 2 were added into the medium for 24 h, respectively. Using PGF 2? to inhibit PPAR? activation via activation of MAP kinase. We employed the flow cytometry analysis to quantitatively analyze the apoptosis of VSMCs. Also, we measured and analyzed the discrepancies of the expression of p53 and bcl-2 between every groups. RESULTS: During the process of apoptosis induced by PPAR?,the expression of P53 protein increased (P
