Effects of insulin and glucose on secretion of tissue-type plasminogen activator and its inhibitor-1 in hypoxic vascular endothelial cells
- VernacularTitle:胰岛素和葡萄糖对缺氧血管内皮细胞分泌tPA和PAI-1抗原的影响
- Author:
Zhongcai FAN
;
Zhi ZENG
;
Xiaoqing LIU
;
Yuan FANG
;
Xingbin LIU
- Publication Type:Journal Article
- Keywords:
Insulin;
Glucose;
Endothelial cells;
Plasminogen activators;
Plasminogen activator inhibitor 1
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of insulin and glucose on tissue-type plamingen activator (tPA) and its inhibitor-1 (PAI-1) secretion in cultured human endothelial cells. METHODS: Human endothelial cell line ECV-304 was cultured with glucose and/or insulin at different concentrations with or without hypoxic exposure. RESULTS: The tPA, PAI-1 secretion and ratio of tPA/PAI-1 increased in endothelial cells during hypoxia. Insulin and glucose increased the tPA and PAI-1 secretion in endothelial cells exposed to hypoxia, and increase in tPA/PAI-1 ratio was also observed at 4 h and 8 h. CONCLUSION: Hypoxia stimulates the release of tPA and PAI-1. Insulin and glucose also stimulate the tPA and PAI-1 secretion during hypoxia.
