Expression of novel gene SNC66 in gastric cancer tissue and normal gastric mucosa
- VernacularTitle:原位检测新基因SNC66在胃癌组织与正常胃粘膜中的表达
- Author:
Hangui WU
;
Shu ZHENG
- Publication Type:Journal Article
- Keywords:
In situ hybridization;
Reverse transcriptase polymerase chain reaction;
Gene, SNC66;
Stomach neoplasms
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the expression level of gene SNC66 in gastric cancer tissues, and investigate the relationship between the expression of SNC66 and the pathogenesis of gastric cancer.METHODS: Paired normal and cancerous paraffin-embeded tissues from 20 gastric cancer patients were tested by in situ hybridization (ISH) and in situ reverse transcription and polymerase chain reaction (ISRT-PCR)using ?-actin as a control.RESULTS: ISH and ISRT-PCR both showed that SNC66 were all strong positive expressed in 20 normal gastric mucosa.But in 20 gastric cancer tissues,the ISH showed that 8 were negative,8 were weak positive and 4 were strong positive. The ISRT-PCR showed that 6 were negative,9 were weak positive and 5 were strong positive when the PCR cycled 10 times;4 were negative and 16 were strong positive when the PCR cycled 25 times. CONCLUSION: There was significant expression reduction or defect of SNC66 in gastric cancer tissues.SNC66 should be further investigated as a gastric cancer negative relative gene.
