The Effect of Mesenchymal Stem Cells Transplantation on Heart Function of Rat Myocardial Infarction
- VernacularTitle:骨髓间充质干细胞心肌移植对大鼠心梗后心功能的影响
- Author:
Tao TANG
;
Jianguo HU
;
Jinfu YANG
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cell;
Transplantation;
Myocardial infarction;
Cardiac function;
SERCA
- From:
Journal of Chinese Physician
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of mesenchymal stem cells transplantation on heart function and the expression of Ca 2+ -ATPase (ERCA) mRNA after rat myocardial infarction. Methods Wistar inbred rat mesenchymal stem cells were cultivated, proliferated and labeled in vitro. Myocardial infarct models were set up by liquid nitrogen cryoinjuring the free wall of left ventricle. 4 weeks later, 2?10 6 mesenchymal stem cells or cold D-Hanks solution were injected into several different points of infarcted myocardial area of experiment group and control group rats. In addition, 5 Wistar inbred rats served as normal group only got thoracotomy twice. 4 weeks after transplantation, all rats received cardiac function test and specimens from infarcted area were got for the expression assay of SERCA2 mRNA by RT-PCR. Results Mesenchymal stem cells were alive at 1, 2 and 4 weeks after transplantation and had a trend toward differentiation and maturation in vivo. Traumatic dynamic measurement was applied to assay heart function by Buxco system, which showed that SLVP and +dp/dt were the highest in normal group, next in experiment group, and the lowest in control group, which LVEDP was the lowest in normal group, next in experiment group, and the highest in control group. The expression level of SERCA2 mRNA in control group was much lower than that in normal group(0.988?0.004 VS 1.824?0.009,P
