Effect of Adenovirus-mediated p27kip1 Gene Transfer on Cell Cycle of Esophageal Carcinoma Cells
- VernacularTitle:腺病毒介导的p27kip1基因转移对食管癌细胞周期的影响
- Author:
Weiguo ZHANG
;
Qingming WU
;
Qiang TONG
- Publication Type:Journal Article
- Keywords:
p27kip1;
Gene transfer;
Esophageal carcinoma;
Cell cycle
- From:
Journal of Chinese Physician
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effect of p27kip1 gene transfer mediated by adenovirus vector on the cell cycle of esophageal carcinoma cells. Methods The recombination adenoviral vector carrying p27kip1 gene (Ad-p27kip) was transfected to esophageal carcinoma Eca9706 cells. The effect of p21kip1 gene transfer on the cell growth suppression was measured by MTT method. Expression of p27kip1 was detected by immunocytochemical technique. Cell cycle was analysed by flow cytometry(FCM). Results After Eca9706 cells were transfected with Ad-p27kip1, p27kip1 expression increased, the cell growth was significantly inhibited and the ratio of G 0/G 1 phase cells increased and the cell number of G 2/M and S phases decreased. Conclusion p27kip1 gene transfer mediated by adenovirus can significantly inhibit the growth of esophageal carcinoma cells and cause G 1 phase blocking.
