The establishment of a Tet-on transfected human hepatoma HepG2 cell line regulated by doxycycline
- VernacularTitle:受强力霉素调控表达的人肝癌HepG2细胞系的建立
- Author:
Donghua LI
;
Xiaoping CHEN
;
Xiaoming QUAN
- Publication Type:Journal Article
- Keywords:
Liver neoplasms;
Gene expression regulation, neoplastic;
Cell line
- From:
Chinese Journal of General Surgery
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish human hepatic carcinoma HepG2 cell line in which the expression of exogenous gene could be controlled by doxycycline. Methods The expression plasmid pTet-on was transfected into hepG2 cells by lipofectamine 2000.The stably transfected positive clones were screened with G 418 and subcultured. The expression level of luciferase induced by doxycycline at a concentration of 1 ?g/ml in stably transfected cells was detected one by one by transient transfection of plasmid pTRE-luc into the stably transfected cells. Results The basal expression activity of luciferase in cells of clone No 6 was 87, and the activity was 16764 after induction with doxycycline. Conclusion The HepG2/Tet-on cell line was a regulatory human hepatoma cell line, which is useful for the study of carcinogenesis and gene therapy of hepatic carcinoma.
