Comparative Study on Two Mouse Models of Sclerotic Skin
- VernacularTitle:两种小鼠皮肤硬化模型的对比研究
- Author:
Kai ZHAO
;
Wenzhen TU
- Publication Type:Journal Article
- Keywords:
Scleroderma, circumscribed;
Bleomycin;
Mice, inbred C3H;
Mice, inbred BALB c;
Models, animal
- From:
Chinese Journal of Dermatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the mouse model of sclerotic skin. Methods The sclerotic skin was induced by local injections of bleomycin (BLM) in C3H and BALB/c mice. The injection solution was prepared with BLM at the concentration of 200?g/mL in PBS. In the test group 0.1 mL BLM solution was injected daily into the back of the mice for 3 weeks. For the control mice same amount of PBS was injected daily for 3 weeks. After 3 weeks, the histology of the skin and lungs was compared between the different groups, also the changes of skin thickness and quantity of collagen. Results After the treatment of BLM sclerotic skin was observed in both C3H and BALB/c mice, in which the thickness of skin and the quantity of collagen (6 mm ? 6 mm) were higher than those in the control mice (P
