Study on the Expression of Interferon-gamma in Experimental Murine Systemic Scedosporum Apiospermum Infection
- VernacularTitle:系统性尖端赛多孢感染小鼠干扰素?表达的研究
- Author:
Daofan LIU
;
Yuechen ZHENG
;
Jiawen LI
;
Tao LU
;
Jingsi ZENG
;
Yanqing WU
- Publication Type:Journal Article
- Keywords:
Scedosporium;
Mycoses;
Interferon type Ⅱ;
Animal testing alternatives
- From:
Chinese Journal of Dermatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression and the role of interferon-gamma (IFN-?)in murine systemic infection of Scedosporum apiospermum. Methods The murine models of systemic Scedosporum apiospermum infection was established by inoculation of the pathogenic fungi. ELISA and RT-PCR were applied to detect the expression level of IFN-? protein and mRNA in spleens, respectively. Colony formingunit (cfu) of infected kidneys was determined with the plating dilution method. The mean survival time (MST) of the mice was also recorded. Results IFN-? levels in lethal infection group were lower than those of the normal controls (P 0.05 on day 7). IFN-? levels of sublethal infection group were higher than those of normal controls (P 0.05 on day 7). But the IFN-? levels were significantly lower in the immunosuppressed group than those in the sublethal infection group (P
