Simultaneous Determination of Oleanolic Acid and Ursolic Acid in Hedyotis Diffusa with HPLC
- VernacularTitle:高效液相色谱法同时测定白花蛇舌草中两组分的含量
- Author:
Xingen LUO
;
Linlan JIANG
;
Shujin ZHAO
- Publication Type:Journal Article
- Keywords:
Hedyotis diffusa;
oleanolic acid;
ursolic acid;
determination of content;
HPLC
- From:
China Pharmacy
1991;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a RP-HPLC method for simultaneous determination of oleanolic acid and ursolic acid in Hedyotis diffusa.METHODS:The optimum HPLC conditions were as follows:Nucledure C 18 column(4.6mm?150mm);mobile phase:acetonitrile-potassium phosphate,pH=7.84(45∶55);detection wavelength:220nm.RESULTS:The average recoveries of oleanolic acid and ursolic acid were(96.79?2.42)%and(97.15?2.33)%respectively.CONCLUSION:The met_ hod could detect oleanolic acid and ursolic acid in Hedyotis diffusa simultaneously.It could be used in the quality control of the herb.
