Effects and mechanism of magnetic microsphere combination of cisplatin with micron ferric oxide particle on human hepatoma cell in vitro
- VernacularTitle:纳米及微米磁微粒磁性顺铂微球对肝癌细胞的影响及机理
- Author:
Yueming HE
;
Xinsheng LU
;
Zhongli AI
;
Zhisu LIU
;
Pingkan WANG
- Publication Type:Journal Article
- Keywords:
CARCINOMA,HEPATOCELLULAR/drug ther;
CISPLATIN/ther use;
CISPLATIN/admin;
NANO;
MICROSPHERE;
TUMOR CELLS,CULTURED
- From:
Chinese Journal of General Surgery
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and mechanism of magnetic microsphere combination of cisplatin with nano or micron ferric oxide particle on human hepatoma cell(HHC). Methods A human hepatoma cell line BEL-7402 was used in this study, 0.04?g/ml～8?g/ml of cisplatin(CDDP), cisplatin nano-ferric oxide magnetic microsphere(nCDDPmm) and micro-ferric oxide magnetic microsphere(mCDDPmm) were administered to the culture solution for culturing the BEL-7402 cell as the experimental groups,and in the control groups were administered with cisplatin 0?g/ml and magnetic microsphere without cisplatin respectively. The optic density of viable cell, cytotoxity index, growth curve of cell, cell cycle, proliferation index and apoptosis were assayed by MTT method, cell count and flow cytometry respectively. Results (1)The viable cell's optic density decreased and the cytotoxity index increased in human hepatoma cell following increasing dosages of CDDP, nCDDPmm and mCDDPmm in culture solution,presenting a dose-dependent manner(r value of three drugs were 0.95, 0.91 and 0.89 respective, P 0.05)between nCDDPmm and mCDDPmm. (3)Apoptosis rate of hepatoma cell increased ( P
