Significance of expression of angiopoietin mRNA in the tissue of in-situ implanted hepatoma
- VernacularTitle:促血管生成素在原位种植肝癌组织中的表达及其意义
- Author:
Xueqiang LIU
;
Hengrong WAN
;
Haisheng CHEN
;
Li PENG
- Publication Type:Journal Article
- Keywords:
ANGIOPOIETIN;
LIVERNEOPLASMS/pathol;
GENE ERPRESSION;
LIVER NEOPLASMS/blood supply;
LIVERNEOPLASMS,EXPERIMENTAL;
DISEASE MODELS,ANIMAL
- From:
Chinese Journal of General Surgery
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship of expression of angiopoietin gene and angiogenesis in the in-situ implanted hepatoma in rats. Methods Forty Wistar rats were used to establish the in-situ implanted hepatoma model by implanting Walker256 hepatoma cell line. The number of microvessel density(MVD) in hepatoma was calculated . The expression of angiopoietin mRNA was observed by in-situ hybridization method. Results The MDV in the implanted hepatoma in the first day of 1, 2 and 3 week postimplantatively were (15?4.3)/Hp,(17?3.6)/Hp,(45?7.8)/Hp respectively. The amount of MDV and expression of Ang-1 mRNA were increased significantly and correlated with MDV in the hepatoma tissues and non-hepatoma tissues,and no significant difference between the two types of tissues. Expression of Ang-2 mRNA was not seen in non-hepatoma tissues,but in hepatoma tissues,the expression of Ang-2 was obvious. Conclusions Angiopoietin-2 mRNA may play a role in the angiogenesis of the in-situ implanted hepatoma.
