Effects of sterigmatocystin on IL- 2 and IFN - ? secretion and expression in murine spleen cells in vitro
- VernacularTitle:杂色曲霉素对小鼠脾细胞IL-2及IFN-?分泌和表达的影响
- Author:
Lingxiao XING
;
Xianghong ZHANG
;
Yuehong LI
;
Xia YAN
;
Junling WANG
;
Fengrong WANG
- Publication Type:Journal Article
- Keywords:
Sterigmatocystin;
Spleen;
Interleukin 2;
Interferon type Ⅱ;
Reverse transcriptase polymerase chain reaction
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effects of sterigmatocystin (ST) on IL-2 and IFN-? expression and secretion in murine spleen cells in vitro. METHODS: The secretion and expression of IL-2 and IFN-? in murine spleen cells after ST pretreatment at five different dosages (0.125 mg/L, 0.25 mg/L, 0.5mg/L, 1mg/L, 2mg/L) were studied with ELISA and semi-quantitative RT-PCR method, respectively. RESULTS: Pretreatment of murine spleen cells in vitro with ST at five different dosages affected the IL-2 and IFN-? secretion at protein level and expression at mRNA level of the treated cells. The effects varied dependently to the ST dosage. At relatively lower dosages, ST induced the expression of IL-2 and IFN-? in murine spleen cells, while at relatively higher dosages, inhibitory effects were found, with the most significant inhibitory effects seen in ST 1 mg/L group. CONCLUSION: ST affected the secretion and expression of IL-2 and IFN-? in treated murine spleen cells. At relatively lower dosage, ST induced IL-2 and IFN-? secretion and expression, while at relatively higher dosages, inhibitory effects appeared.
