Effects of ?_3 integrin on adhesion and migration of vascular smooth muscle cells induced by growth factor
- VernacularTitle:?3整合素在生长因子促血管平滑肌细胞粘附和迁移中的作用
- Author:
Yubin HAO
;
Jinkun WEN
;
Mei HAN
- Publication Type:Journal Article
- Keywords:
Integrin ?_3;
Platelet-derived growth factor;
Muscle;
smooth;
vascular;
Adhesion;
Cell movement;
Gene expression
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of platelet-derived growth factor (PDGF) on ?_3 integrin gene expression and the role of ?_3 integrin on adhesion, migration and proliferation of vascular smooth muscle cells (VSMC) induced by PDGF. METHODS: ?_3 integrin gene expression was detected by RT-PCR. After ?_3 integrin extracellular domain was blocked, VSMC adhesion, migration and proliferation were measured by adhesion assay, a wound-culture model and [~3H]-TdR incorporation, respectively. RESULTS: After the interaction between ?_3 integrin and extracellular matrix was blocked, VSMC proliferation was inhibited in some degree and the rate of [~3H]-TdR incorporation into VSMC decreased 39%. The cell adhesion and migration were significantly inhibited when 10 mg/L anti-?_3 integrin antibody was added (P
