Screening of the differentially expressed genes in lymphocytes of patients with unstable angina with suppression subtractive hybridization
- VernacularTitle:抑制性消减杂交筛选不稳定性心绞痛相关基因
- Author:
Bin ZHANG
;
Xueqing LIANG
;
Xiyong YU
;
Jianzhang FENG
;
Lijun JIN
;
Taiming DONG
;
Handong WU
;
Tao HUANG
;
Hongtao LIAO
- Publication Type:Journal Article
- Keywords:
Angina;
unstable;
Suppression subtractive hybridization;
Gene expression
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To screen and identify the differentially expressed genes in lymphocytes of patients with unstable angina in order to find the molecular mechanism of unstable angina. METHODS: Suppression subtractive hybridizations (SSH) and dot blot hybridizations were performed to screen the relatively differentially expressed genes in lymphocyte RNA between the patients with unstable angina pectoris and stable angina pectoris. The obtained expressed sequence tags (ESTs) were used as probes to perform Reverse Northern blot with forward and reverse suppression products. And the positive ESTs were performed RNA slot hybridization with unstable and stable angina group. The obtained ESTs were sequenced and analyzed using BLAST (nr) at NCBI. RESULTS: Three up-regulated ESTs in the unstable angina group, and one down-regulated EST in the stable angina group were obtained. All of them are sequences of known genes. CONCLUSION: All these ESTs may be associated with the unstablization of plaque of coronary artery in patients with unstable angina.
