Influence of serum contained Tongmai herbal medicine on intracellular calcium concentration and L- type calcium channel in anoxic myocardial cells
- VernacularTitle:通脉汤含药血清对缺氧心肌细胞游离钙及L型钙通道的作用
- Author:
Tong LI
;
Ming SUN
;
Hongyan ZHOU
;
Kaichao LONG
;
Suixin LIU
;
Fei HE
- Publication Type:Journal Article
- Keywords:
Myocardium;
Anoxia;
Calcium overload;
Tongmai herbal medicine
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the influence of serum contained Tongmai herbal medicine on calcium overload of the anoxic myocardial cells. METHODS: Serum contained Tongmai herbal medicine was prepared with the serum pharmacological method and the model of anoxic myocardial cells was established. The intracellular free calcium concentration and the expression of the L-type calcium channel were determined with Fura-2/AM and RT-PCR, respectively. RESULTS: The serum contained Tongmai herbal medicine and levo-anlodipine decreased the intracellular free calcium concentration in the anoxic myocardial cells. The serum contained Tongmai herbal medicine also suppressed the expression of L-type calcium channel in the anoxic myocardial cells, but levo-anlodipine had no effect on the expression of L-type calcium channel. CONCLUSION: Tongmai herbal medicine can attenuate calcium overload in anoxic myocardial cells.
