Effects of hydrogen peroxide on intracellular free calcium concentration in cultured neonatal rat cardiomyocytes and its antagonism by taurine
- VernacularTitle:过氧化氢对乳鼠心肌细胞内游离钙浓度的影响及牛磺酸的拮抗作用
- Author:
Hexiang CHENG
;
Guoliang JIA
;
Rongqing ZHANG
;
Haichang WANG
;
Bing LIU
;
Xuyang FENG
;
Tao HU
- Publication Type:Journal Article
- Keywords:
Myocardium;
Hydrogen peroxide;
Calcium;
Taurine
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To examine the effects of hydrogen peroxide on intracellular free calcium concentration([Ca~(2+)]i) in cardiomyocytes and its antagonism by taurine. METHODS: A cell culture model of neonatal rat cardiac myocytes was used. There were four groups, control group, hydrogen peroxide (H_2O_2) group, H_2O_2+taurine (simultaneously) group,and H_2O_2+taurine (in sequence) group. Confocal microscope was used with Fluo-3/AM as calcium indicator to detect changes of [Ca~(2+)]i immediately and 15 minutes after H_2O_2 intervention, respectively. RESULTS:The intracellular fluoresence intensity of singular cardiomyocyte in H_2O_2 group was significantly higher than the control group 15 minutes after intervention (P
