Protective effect of HSP_70 on injuried myocardial cells induced by H_2O_2
- VernacularTitle:热休克蛋白70对H_2O_2诱发大鼠心肌细胞凋亡的保护作用
- Author:
Weihua ZHANG
;
Lina WANG
;
Quanfeng LI
;
Changqing XU
- Publication Type:Journal Article
- Keywords:
Heat-shock proteins 70;
Hydrogen peroxide;
Apoptosis;
Myocardium;
Rats
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of heat-shock protein 70(HSP_(70)) in the protection of myocardial cells against ischemic injury. METHODS: Myocardial cells were cultured in vitro. HSP_(70) was induced by hyperthermia.H_2O_2-injured myocardial cells were divided into different groups. Flow cytometry, DNA Ladder and biochemistry methods were employed to observe the myocardial cells of different groups. RESULTS: Immunohistochemistry showed hyperthermia induced the up-regulation of HSP_(70) in myocardial cells. Apoptotic rate, activity analysis of cytochrome C and succinic dehydrogenase in H_2O_2-injuried and HSP_(70)-protected groups were obviously different. Electron micrograph shomed hyperthermia alliviated myocardial cell injury induced by H_2O_2. CONCLUSION: HSP_(70) delays apoptosis and protects against H_2O_2-induced myocardial cell injury.
