Effect of adenoviral vector containing AT1 receptor antisense cDNA on migration of vascular smooth muscle cells
- VernacularTitle:腺病毒介导的反义AT1R转染对血管平滑肌细胞迁移的影响
- Author:
Mingli TU
;
Yuanchao TU
;
Zheng CAO
;
Weimin WANG
;
Xiaping CHEN
;
Xiaoxun WANG
;
Hanqin WANG
;
Jianing WANG
;
Guiyuan YANG
- Publication Type:Journal Article
- Keywords:
Angiotensin Ⅱ;
Adenoviruses;
human;
Muscle;
smooth;
vascular;
Cell movement
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To evaluate the role of human angiotensin Ⅱ(AngⅡ) type Ⅰ receptor (AT1R) antisense cDNA (ahAT1) on migration of cultured artery smooth muscle cells (VSMCs). METHODS: Two recombinant adenoviral vectors, Ad/CMV. ahAT1 containing full length antisense cDNA targeting to human AT1R mRNA, and Ad/CMV.LacZ containing LacZ called report gene, were constructed by orientation clone technology and homologous recombination, and then were used to transfect VSMCs in vitro. AT1R expression detected by RT-PCR and immunohistochemistry, and migration of VSMCs measured by Boyden's Chamer methods, were compared between transfected and non- transfected VSMCs. RESULTS: Forty-eight hours after Ad/CMV. ahAT1 transfection, the level of AT1R mRNA decreased markedly (50% of control group), and AT1R protein expression was significantly less (P
