Actions of vecuronium and atracurium on adult and embryonic-type nicotinic acetylcholine receptor at skeletal muscle cell membrane
- VernacularTitle:维库溴铵和阿曲库铵对骨骼肌细胞膜上成人型和胚胎型乙酰胆碱受体的作用
- Author:
Xuelian ZHAO
;
Xinliang ZHUANG
;
Baazhong YANG
- Publication Type:Journal Article
- Keywords:
Atracurium;
Vecuronium bromide;
Muscle,skeletal;
Cell membrane;
Receptors,cholinergic
- From:
Chinese Journal of Anesthesiology
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate and compare the actions of vecuronium and atracurium on adult and embryonic-type nicotinic acetylcholine receptors (?-and ?-nAChR) at skeletal muscle cell membrane. Methods The adult and embryonic nAChRs were heterologously expressed in HEK 293 cells. Peak currents induced by actions of vecuronium and atracurium at ?-and ?-nAChR were recorded in HEK 293 cells, using whole cell patch clamp technique. Results Vecuronium and atracurium competitively inhibited ?-and ?-nAChR in HEK 293 cells. The inhibitor concentrations for half-maximal response (IC50) for vecuronium and atracurium ate-nAChR were (8.3 ? 2.6) ?mol/L and (24.2 ? 10.5) ?mol/L respectively; The IC50 values for vecuronium and atracurium at e-nAChR were (55.0 44 28.4) ?mol/L and (183.2 ? 39.2) ?mol/L respectively.Conclusion According to IC50 values for both adult and embryonic type nAChR, vecuronium is more potent than atracurium on e-and 7-nAChR. Embryonic nAChR is less sensitive to vecuronium and atracurium than adult-type nAChR.