The Effect of Mesenchymal Stem Cells Transplantation on Angiogenesis and Secretion of Cytokines After Rat Myocardial Infarction
- VernacularTitle:骨髓间充质干细胞心肌移植对大鼠心梗后血管新生和细胞因子分泌的作用
- Author:
Jinfu YANG
;
Tao TANG
;
Jianguo HU
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cell;
Transplantation;
Angiogenesis;
Myocardial infarction;
Cytokines
- From:
Journal of Chinese Physician
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of mesenchymal stem cells transplatation on angiogenesis and secretion of cytokines after rat myocardial infarction. Methods Harvested rat mesenchymal stem cells were cultured and labeled in vitro. And at the same time, myocardial infarct model was set up by liquid nitrogen cryoinjuring the left ventricular free wall. 4 weeks later, 2?10 6 mesenchymal stem cells or cold D-Hanks liquid were injected into several different points of infarcted area. At 1, 2 and 4 weeks after mesenchymal stem cells transplatation, specimens were acquired from infarcted area. Then cell morphology and capillary density were measured, and vascular endothelial growth factor(VEGF) and transforming growth factor ?(TGF-?) expression levels were assayed by RT-PCR. Results Mesenchymal stem cells were alive at 1, 2 and 4 weeks after transplantation and had a trend toward differentiation and maturation in vivo. The number of capillary vessels in per infarcted area (0 1 mm 2) in experiment group increased compared with control group. And the number of capillary vessels in experimental group had a trend to decrease in experiment group(P
