The Reverse Effect of Chinese Medicine MBC on the Drug-resistance of K562/ADM Cells
- VernacularTitle:中药MBC逆转K562/ADM细胞的体外研究
- Author:
Chunyan TIAN
;
Fengchun ZHANG
;
Yumei LIN
- Publication Type:Journal Article
- Keywords:
Chinese traditional medicine;
Malignant neoplasms;
Multidrug resistance;
Reversal;
P-glycoprotein(P-gp)
- From:
Journal of Chinese Physician
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the reverse effect and mechanism of the extracted solution of chinese medicine MBC on the drug-resistance of K562/ADM in vitro. Methods MTT assay was used to determine the IC50 of ADM on K562/ADM cell line and the reverse multiples of the drug-resistance before and after MBC treatment. Flow cytometry(FCM) was applied to assess the intracellular concentration of Rh123. Results 8?10 -1 ?g/ml MBC increased cytotoxic effect of ADM on 562/ADM cells, the reverse multiple was 6 28, and MBC could increase the intracellular concentration of Rh123. Conclusion MBC could increase killing effect of ADM on the drug-resistant K562/ADM cell and partly reverse the drug-resistance of K562/ADM cell.
