Effects of Rosiglitazone on GLUT4 Protein Translocation in Skeletal Muscle of Insulin Resistance Rat
- VernacularTitle:罗格列酮及饮食干预对胰岛素抵抗大鼠骨骼肌GLUT4转位的影响
- Author:
Xiaolin CHEN
;
Huimin BI
- Publication Type:Journal Article
- Keywords:
Rosiglitazone;
Insulin resistance;
Skeletal muscle;
Glucose transport-4
- From:
Journal of Chinese Physician
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effects of rosiglitazone on glucose transport-4(GLUT4) protein translocation in skeletal muscle of insulin resistance rats. Methods 38 male sprague dawley rats were randomized to received high fat or standard chow diet for 8 weeks, feeding high-fat diet to induce insulin resistance in skeletal muscle of rats. 25 high-fat-fed rats were randomly treated with rosiglitazone(1mg/kg), standard chow diet, or placebo(high-fat diet) for 4 weeks. Plasma concentrations of glucose and insulin, weight and viscero-fat in all rats were measured. The GLUT4 level in the cell membrane of isolated rats skeletal muscle was detected by western blotting analysis. Results Insulin-stimulated translocation of GLUT4 to the plasma membrane in skeletal muscles of high fat-fed rats was significantly lower than that of standard chow-fed rats(52 72%,P
