Infected endophthalmitis diagnosed by multiplex polymerase chain reaction
- VernacularTitle:多重聚合酶链反应快速诊断感染性眼内炎的研究
- Author:
Hong LU
;
Huaijin GUAN
;
Qirong GONG
- Publication Type:Journal Article
- Keywords:
Polymerase chain reaction;
Endophthalmitis/diagnosis;
Endophthalmitis/microbiology;
Bacteria;
Fungi
- From:
Chinese Journal of Ocular Fundus Diseases
1999;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the rapid diagnosis of bacterial and (or) fungal endophthalmitis by multiplex polymerase chain reaction (MPCR). Methods MPCR was performed to detect the DNA segment of bacteria and (or) fungi from standard strains and 41 samples of intraocular fluid or vitreous from 38 patients (3 with double eyes and 35 with single), and the results were compared with the cultured bacteria and fungi. Results Five hours after detected by MPCR, bacteria and (or) fungi in 34 out of 41 samples (82.9%) from patients were detected,including bacteria in 26,fungi in 6,and both bacteria and fungi in 2. The positive rate of MPCR was obviously higher than the cultured ones( ? 2 =9.60, P
