Study on Microsatellite DNA Loss of Heterozygosity in p16 Gene of Psoriatic Keratinocytes
- VernacularTitle:银屑病角质形成细胞p16基因微卫星杂合性缺失研究
- Author:
Hongwen LI
;
Lei YONG
;
Ge GAO
;
Yi DING
;
Shiqi WANG
- Publication Type:Journal Article
- Keywords:
Psoriasis;
Genes,p16;
Microsatellite repeats;
Loss of heterozygosity
- From:
Chinese Journal of Dermatology
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To detect the loss of heterozygosity(LOH)frequencies of microsatellite loci D9S171and D9S1604in p16gene of psoriatic keratinocytes,and to study the correlation between mi-crosatellite LOH of p16gene and the development of psoriasis.Methods By the use of polymerase chain reaction(PCR)-denaturing polyacrylamide gel electrophoresis-silver staining,LOH was detected with23sam-ples of keratinocytes from psoriatic lesions and non-lesion skin.Results LOH was identified at loci D9S171and D9S1604in5and10out of23keratinocyte samples from LOH-exhibited psoriatic lesions,and in2and3of keratinocyte samples from psoriatic non-lesion skin,respectively.The frequency of LOH at D9S1604was significantly higher in psoriatic lesion samples than that in psoriatic non-lesion skin(P
