Influence of Psoriatic T Lymphocytes on Epidermal c-myc,bcl-2 and p53 Protein Expression in vitro
- VernacularTitle:银屑病患者T细胞对表皮c-myc、bcl-2及p53蛋白表达影响的实验研究
- Author:
Xinhua LI
;
Kaiming ZHANG
;
Yuying KANG
- Publication Type:Journal Article
- Keywords:
Psoriasis;
T-lymphocytes;
Proto-oncogene proteins c-myc;
Proto-oncogene proteins c-bcl-2;
Proteins p53
- From:
Chinese Journal of Dermatology
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of abnormal epidermal proliferation induced by psoriatic T lymphocytes.Methods Skin organ culture was established with psoriatic T cells mixed with epi-dermal cells.The expressions of c-myc,bcl-2and p53protein were examined with immunohistochemical method in epidermis before culture,on the3rd day and the6th day after culture.Results Significant up-regulation of c-myc and p53protein was found in psoriatic lesions,but bcl-2protein expression was rarely observed.The expressions of those proteins were normal in non-lesional psoriatic skin.The p53protein ex-pression was increased in normal skin and non-lesional psoriatic skin on the3rd day after culture with psori-atic T cells,and c-myc protein expression was enhanced while bcl-2was decreased on the6th day of co-culture.There was no significant difference of those proteins' expression between normal skin and non-lesion-al psoriatic skin stimulated by psoriatic T cells.Conclusions The abnormal expressions of c-myc,bcl-2and p53protein play an important role in abnormal epidermal proliferation and differentiation in psoriasis.Psoriatic T lymphocytes can influence c-myc,bcl-2and p53protein expression in normal skin and non-le-sional psoriatic skin.Pathogenic T cells rather than keratinocytes might be vital for initiation of psoriasis.
