Study of the relationship of alteration and expression of p16 gene and p16 protein on the hilar cholangiocarcinoma
- VernacularTitle:肝门胆管癌中p16基因突变及蛋白表达异常的研究
- Author:
Ge GAO
;
Junmin WEI
;
Shengquan ZOU
;
Fazu QIU
- Publication Type:Journal Article
- Keywords:
BILE DUCT NEOPLASMS/pathol;
NEOPLASM METASTASIS;
GENE p16;
GENE EXPRESSION
- From:
Chinese Journal of General Surgery
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of genetic alteration(homozygous deletion and point mutation)and expression of p16 gene and p16 protein on hilar cholangiocarcinoma(HCGC) . Methods Genetic alteration and expression of p16 protein were detected by polymerase chain reaction single strand comformation polymorphism (PCR-SSCP) and immunohistochemical method in 36 HCGC tissues. Results p16 gene revealed alteration in 21 of 36 HCGC tissues (58.3%),among which 8 had homozygous deletion and 13 had point mutation. In HCGC tissues, 8 revealed no p16 protein expression and 10 showed low level expression of p16 protein. Conclusions The alteration of p16 gene and abnormal expression of p16 protein are significantly correlated with the biological behavior and clinical staging of HCGC,and may be helpful to evalute the malignant degree of HCGC and the patients prognosis.
