Role of potassium channels in the regulation of intracellular free Ca~(2+) concentration of pulmonary artery smooth muscle cells in rats
- VernacularTitle:钾通道对大鼠肺动脉平滑肌细胞[Ca~(2+)]_i的调节
- Author:
Zaiwen FAN
;
Zhenxiang ZHANG
;
Yongjian XU
- Publication Type:Journal Article
- Keywords:
Potassium channels;
Calcium;
Pulmonary artery;
Muscle,smooth
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of potassium channels in the regulation of intracellular free calcium concentration (_i) of pulmonary artery smooth muscle cells (PASMCs) in rats. METHODS: The fluorescence Ca 2+ indicator Fura-2/AM was used to observe _i of rat PASMCs in normal and chronic hypoxic condition. The influences of potassium channels on PASMCs proliferation were assessed by MTT assay. RESULTS: 1. In normoxic condition,_i was (156.91?8.60) nmol/L,and in hypoxic condition,_i was (294.01?16.81) nmol/L. 2. In normoxic condition,the voltage-dependent K +-channel antagonist 4-aminopyridine (4AP),but not the Ca 2+ -activated K +-channel antagonist tetraethylammonium (TEA) and the ATP-sensitive K +-channel antagonist glibenclamide (Glib) increased _i. 3. In hypoxic condition,4AP and TEA caused the rise in _i [(422.41?24.28) nmol/L,(380.84?11.02) nmol/L,respectively],but Glib had no effect on _i. 4. MTT assay showed that 4AP increased the value of absorbing light degree ( A value) in normoxic and hypoxic condition (0.582?0.062,0.873?0.043,respectively, P
