Effects of LPS,TNF-? and IL-1? on cyclooxygenase-2 expression in human umbilical vein endothelial cells
- VernacularTitle:LPS、TNF-?、IL-1?对人脐静脉内皮细胞COX-2表达的影响
- Author:
Jianchun WANG
;
Baoling MAO
;
Guisheng QIAN
- Publication Type:Journal Article
- Keywords:
Human umbilical vein endothelial cells;
Prostaglandin-endoperoxide synthase;
Prostaglandins
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore effects of LPS,TNF-?,IL-1? on cyclooxygenase-2(COX-2) expression and prostaglandins(PGs) production in human umbilical vein endothelial cells (HUVEC). METHODS: HUVEC were stimulated with lipopolysaccharide(LPS),TNF-?,IL-1? for 24 hours. Using in situ hybridization,reverse-transcription polymerase chain reaction and immunohistochemistry, COX-2 expression in both mRNA and protein level were observed and prostaglandins in culture medium were measured. RESULTS: Resting HUVEC expressed a little COX-2 mRNA. Expression of mRNA and protein of cyclooxygenase-2 increased significantly post-stimulation with LPS,TNF-?,IL-1? and PGs raised markedly at the same time. CONCLUSIONS: Resting HUVEC expressed a little COX-2 mRNA, inflammatory stimuli induced COX-2 superexpression and PGs production. These results suggested that endothelial cells could involve in inflammation by controlling of COX-2.
