Effect of isoflavone genistein on activation and proliferation of mouse T cells in vitro
- VernacularTitle:染料木黄酮对小鼠T细胞体外活化及增殖的影响
- Author:
Qing WANG
;
Xianhui HE
;
Jingxian ZHAO
;
Zhigang YANG
;
Yaoying ZENG
- Publication Type:Journal Article
- Keywords:
Genistein;
Antigen,CD69;
Receptors,interleukin-2;
T-lymphocytes;
Flow cytometry
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of genistein on activation and proliferation of T cells,and explore the molecular mechanism of genistein. METHODS: Fluorescence conjugated monoclonal antibodies and flow cytometry were used to detect the express of CD69 and CD25 by activated T cells in vitro in response to Concanavalin (ConA )and Phorbol 12,13-dibutyrate(PDB) or T cell proliferation stained by CFSE in response to PDB / Ionomycin or ConA. RESULTS: Genistein inhibited the expression of CD69 and CD25 in activated T cells in response to Con A in a concentration-dependent manner and in response to PDB in a high concentration. Genistein inhibited proliferation of T cells in both groups in a concentration-dependent manner. CONCLUSION: Genistein inhibited activation and proliferation of T cells in vitro in response to polyclonal stimulus,and it may hold potential as a new immunosuppressant.
