Effect of nuclear factor kappa B inhibitor on the responsiveness of pulmonary artery rings to protein kinase C in rats with hypoxia-induced pulmonary hypertension
- VernacularTitle:核因子-?B抑制剂在蛋白激酶C对低氧性肺动脉高压大鼠肺动脉环反应性影响中的作用
- Author:
Huanping ZHANG
;
Yongjian XU
;
Zhenxiang ZHANG
;
Wang NI
;
Shixin CHEN
- Publication Type:Journal Article
- Keywords:
Anoxia;
Hypertension,pulmonary;
Protein kinase C;
NF-kappa B
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of nuclear factor kappa B (NF-?B) inhibitor in the responsiveness of isolated pulmonary artery rings to protein kinase C (PKC) in rats with hypoxia-induced pulmonary hypertension. METHODS: The pulmonary artery rings removed endothelium were prepared from model rats with hypoxia-induced pulmonary hypertension and control rats. The effects of PKC activator PMA (0.5 ?mol/L) time-response cures and NF-?B inhibitor PDTC (0- 1 000 ?mol/L) concentration-response cures on pulmonary artery rings were observed. The responsiveness of each ring was tested by applying a maximally effective concentration of phenylephrine (10 ?mol/L). Data were calculated as relative ratio by the maximally responseness ( P_0 ) setting at 100%,and the relative responseness tensions to PMA and PDTC were derived by dividing by the counts in P_0. t_ 1/2 and T show the time achieving half-maximal response and lasting maxima response to 0.5 ?mol/L PMA,respectively. RESULTS: mPAP and RV/(LV+S) in hypoxia group were greater than those in control group ( P
