Effect of 17?-estradiol on the BMPR-ⅠA,ⅠB gene expression in bone marrow stromal cells
- VernacularTitle:17?-雌二醇对骨髓基质细胞骨形态发生蛋白受体ⅠA、ⅠB mRNA表达的影响
- Author:
Chengliang YUAN
;
Xiaolan JIN
;
Jianhong HOU
;
Qunying LI
;
Yingchu HUANG
- Publication Type:Journal Article
- Keywords:
Estradiol;
Bone marrow stromal cells;
Bone morphogenetic proteins
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of 17?-estradiol (E_2) on the gene expression of typeⅠA and typeⅠB bone morphogenetic protein receptor (BMPR-ⅠA,ⅠB) in rat bone marrow stromal cells exposured to the differentiation medium and to elucidate the effects of E_2 on osteoblastogenesis. METHODS: Adherent bone marrow stromal cells were cultured in differentiation medium containing DEX(10 -7 mol?L -1 ) and 1,25(OH)_2D_3 (10 -9 mol?L -1 ) and different concentrations of E_2. The gene expression of BMPR-ⅠA,ⅠB was quantified by semiquantitative RT-PCR. RESULTS: E_2 evidently inhibited the expression of BMPR-ⅠA mRNA in bone marrow stromal cells.The suppression was dose-dependent. When examined under various concentrations of E_2 (0-10 -6 mol?L -1 ),the expression of BMPR-ⅠA mRNA were decreased from (25.7?2.5)% to(16.3?1.5)%( P
