Effects of cyclooxygenase-2 inhibitor Piroxicam on the growth of colorectal cancer
- VernacularTitle:环氧合酶-2抑制剂吡罗昔康对结肠癌细胞生长的影响
- Author:
Chenggong YU
;
Hui CHEN
;
Zhaomin XU
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Anti-inflammatory agents,non-steroidal;
Cyclooxygenase-2;
Immunohistochemistry
- From:
Chinese Journal of Digestive Endoscopy
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of nonsteroidal anti-inflammatory drugs (NSAIDs) ,cy-clooxygenase-2 ( COX-2 ) inhibitor Piroxicam on the growth of colorectal cancer cells and to evaluate the preventive significance from COX-2 expression and apoptosis. Methods The cell growth of colorectal adenocar-cinoma cell line SW1116 was measured by MTT (3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide) assay, and COX-2 protein expression by Immunohistochemistry and Western Blot. Apoptosis was characterized by DNA fragmentation. Results The results showed that COX-2 inhibitor Piroxicam could restrain the proliferation of SW1116, which had positively related to its concentration. Concentration higher than 1. 0mmol/L showed cytotoxic effects. The inhibition of COX-2 by Piroxicam appeared within 12 hours, but COX-2 protein level recovered within 24 hours, its expression had negatively related to the concentration of Piroxicam. Apoptosis was induced in SW1116 culture with Piroxicam higher than 0. 1mmol/L. Conclusion It can be concluded that cell inhibition effect is associated with Piroxicam-mediated cell apoptosis and inhibition of COX-2 protein expression in SW1116 cell, because the effects of Piroxicam have the concentration and time dependence, in further clinical research its dosage and time of medication should be considered in preventing or treating colorectal cancer.
