Effect of Nao Yi An on VEGF Expression in Rat Brain with Cerebral Hemorrhage
- VernacularTitle:脑溢安对试验性脑出血大鼠血管内皮生长因子表达的影响
- Author:
Saiying WAN
;
Xingqun LI
;
Yihui ZHI
- Publication Type:Journal Article
- Keywords:
Vascular endothelial growth factor;
Cerebral hemorrhage;
Immunohistochemistry;
In situ hybridization;
Nao Yi An
- From:
Journal of Chinese Physician
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study expression change of vascular endothelial growth factor(VEGF) protein and mRNA in rat brain with cerebral hemorrhage and effect of traditional Chinese medicine complex Nao Yi An(NYA) on VEGF expression. Methods The intracerebral hemorrhage(ICH) animal models were made by injecting collagenase type Ⅶstereotaxically into right globus pallidus. The experiment techniques of immunohistochemistry and in situ hybridization were employed to detect the expression variation of VEGF at 3h,6h,12h,1d,4d,7d and 14d after cerebral hemorrhage by positive cell counting as an observation index. Results The VEGF expression in the rat brains was observed at 3h after cerebral hemorrhage, raising at 6h,peaking at 12h and remaining peak until 1d. In the fourth day, VEGF began to decrease and still expressed a little at 7d and 14d.NYA could increase the VEGF expression in rat brains with cerebral hemorhage obviously at 6h ,12h,1d and 4d (P
