Purification and Identification of human Myocardial Troponin T
- VernacularTitle:人心肌肌钙蛋白T的提取和纯化及初步鉴定
- Author:
Guohua ZHOU
;
Jinyue HU
;
Guancheng LI
- Publication Type:Journal Article
- Keywords:
Human cTnT;
Column chromatography;
SDS-PAGE;
Western blot
- From:
Journal of Chinese Physician
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain high pure myocardiac Troponin T from myocardiac tissue. Methods After the myocardiac tissues were homogenized and purified with high salt solution, the Sephacryl S-300HR column chromatography was employed to separate the cTnT from cardiac proteins, and identified them with SDS-PAGE and Western blot. Results The crude cTn was purified by column chromatography, and there appeared three peaks. The second peak was single component, which could react with mouse anti-human Troponin T antibody, and its molecular weight was about 34kD. Conclusions In this experiment, high pure cTnT was obtained, which could pave a way for further research.
