Delayed Protection of Ischemic Preconditioning against Myocardial Ischemia-reperfusion Injury in Rabbits
- VernacularTitle:缺血预处理对家兔缺血再灌注损伤心肌的延迟保护作用
- Author:
Zhengde HUANG
;
Jinwen GE
;
Yusheng ZHANG
- Publication Type:Journal Article
- Keywords:
Ischemia-reperfusion;
Delayed protection;
Ischemic preconditioning;
Adenosine;
Calcitonin gene-related peptide
- From:
Journal of Chinese Physician
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the delayed protection of ischemic preconditioning against myocardial ischemia-reperfusion injury in rabbits. Methods Twenty four healthy rabbits were randomly into sham, ischemia-reperfusion(I/R) and ischemic preconditioning(IP) groups, each group containing 8 rabbits. After 24h IP, rabbits in IP group received 60 min coronary artery occlusion followed by 120 min reperfusion. Myocardial morphological changes were observed by optics microscope and electron microscope, infarction size and the area at risk were determined by triphenyltetrazolium chloride(TTC) and Evan's blue dye, and concentrations of adenosine and calcitonin gene-related peptide(CGRP) in serum were assayed. Results There were a few of cardial fibers ruptured, focus hemorrhage and a mass of inflammatory cell infiltration, and the concentrations of adenosine and CGRP in serum also decreased in rabbits of I/R group. Above changes were improved in rabbits of IP group, and infarction size and the area at risk markedly reduced compared with sham group and I/R group(P
