The Effects of Inhibiting Ubiquitin-proteasome Pathway on DNA Synthesis and Cell Cycle in Gastric Cancer Cell Line SGC-7901
- VernacularTitle:阻断泛素-蛋白酶体通路对胃癌细胞株SGC-7901 DNA合成及细胞周期的影响
- Author:
Weiguo ZHANG
;
Qingming WU
;
Xiaohu WANG
- Publication Type:Journal Article
- Keywords:
Ubiquitin-proteasome;
Gastric cancer;
DNA;
Cell cycle
- From:
Journal of Chinese Physician
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of inhibiting ubiquitin-proteasome pathway(UPP) on DNA synthesis and cell cycle in gastric cancer cell line SGC-7901. Methods The SGC-7901 cells were treated with MG-132, a specific inhibitor of UPP. The effect of MG-132 on cells DNA synthesis was assayed by 3 H-thymidine( 3H-TdR)incorporation. Cell cycle was analysed by flow cytometry(FCM). Results After treated with MG-132, DNA synthesis of SGC-7901 cells was significantly inhibited, the ratio of G 0/G 1 phase cells increased, and the ratio of G 2/M phase and S phase cells decreased. Conclusions MG-132 can significantly inhibit DNA synthesis of gastric cancer cells SGC-7901 and induce G 1 blocking. The data indicated that inhibiting UPP may be a new strategy to treat gastric cancer.
