The effect of reduced glutathione and venous systemic oxygen perfusion on apoptosis and ultrastructure of rat steatotic liver grafts
- VernacularTitle:还原型谷胱甘肽和供氧对伴有脂肪变性的供肝移植后肝细胞凋亡及超微结构的影响
- Author:
Sheng YE
;
Benli HAN
;
Jiahong DONG
;
Jin ZHU
;
Kun LI
;
Yan XIONG
- Publication Type:Journal Article
- Keywords:
Fatty liver;
Liver transplantation;
Glutathione;
Oxygen;
Cell death;
Mitochondria, liver
- From:
Chinese Journal of General Surgery
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of reduced glutathione and venous systemic oxygen perfusion on apoptosis and ultrastructure of hepatocytes in rat steatotic liver grafts. Methods Before liver transplantation grade Ⅱ steatotic liver model was established by a diet consisting of 79% standard diet,20% lard and 1% cholesterol for 6 weeks. In pretreatment group, the donor received intraperitoneal injection of reduced glutathione at a dosage of 500 mg/kg/body weight 3 times a day for 2 days, and intrahepatic venous oxygen perfusion for 6 hours while kept in cold preservation. Results Preconditioning measures in steatotic liver grafts significantly decreased the hepatocytes necrosis (38?10)% vs (17?6)%, P
