The Effects of Asiaticoside on the Growth of Melanoma B16 Cells
- VernacularTitle:积雪甙对黑素瘤细胞生长影响的实验研究
- Author:
Hong SANG
;
Rongzhi NI
;
Xianping SHEN
;
Nan YE
;
Yang CAO
;
Min ZHANG
;
Tong NI
;
Liheng TAN
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Tumor cells, cultured;
Centella asiatica
- From:
Chinese Journal of Dermatology
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of asiaticoside on the growth of melanoma B16 cell cultures in vitro. Methods Melanoma B16 cells were subcultured and the inhibition of cellular growth was investigated. The morphology of the cells was observed after inhibition. The induction of apoptosis by asiaticoside was determined by flow cytometry. Results It was found that asiaticoside could significantly inhibit the growth of B16 cell cultures in vitro in a dose-dependent manner. The annexin-v positive cells were increased, along with that cells intaking R123 marked mitochondria were decreased, and PI positive cells increased, which indicated that cellular apoptosis was induced. Conclusion Asiaticoside plays an inhibitory role in the growth of melanoma B16 cells.
