The Effects of Hairpin RNA on the Expression of Human Papillomavirus E6 Gene
- VernacularTitle:发夹状RNA对人乳头瘤病毒16型E6表达的影响
- Author:
Qili ZHONG
;
Jianjun LI
;
Binzhu CHENG
;
Jie YU
- Publication Type:Journal Article
- Keywords:
Papillomavirus, human;
RNA, messenger;
Plasmids;
Gene expression
- From:
Chinese Journal of Dermatology
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of hairpin RNA on the expression of human papillomavirus E6 gene. Methods Plasmids expressing hairpin RNA, sense RNA and anti-sense RNA were constructed. The recombinants were transfected into cervical cancer cell line, CaSKi. Expression of E6 was detected with semi-quantitative RT-PCR and Western blot. Results Hairpin RNA could significantly reduce the expressions of E6 mRNA and protein, which was more efficient than traditional anti-sense RNA did. Conclusion The RNA interference through hairpin RNA can effectively inhibit the expression of target gene, and can be potentially useful in gene therapy of HPV related tumors.
