Phasic change of water soluble major crystallins with aging in rats
- VernacularTitle:大鼠主要水溶性晶体蛋白在老化进程中的时相变化
- Author:
Wenjie LI
- Publication Type:Journal Article
- Keywords:
Crystallins;
Rats;
Aging
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the correlation between phasic change of the relative quantity of major crystallins with aging in rats. METHODS: Six groups of SD ra ts (age 1 d, 8 d,2 weeks,8 weeks,8 months and 1.5 years) were raised routine ly. Water soluble crystallins were extracted and separated by two-dimensional polyacrylamide gel electrophoresis. After comassize blue staining,the crystallins patterns were sc anned and analyzed. RESULTS: (1) Out of the eighteen water solu ble major rat cr ystallins tested in each group, seven showed gradual phasic changes in relative quantity of crystallins, but there were no significant changes in total quantity of water soluble crystallins. (2) Phasic changes in these crystallins pres ented four different patterns: increasing (?B 4??B 2??A 2??A 1), decreasi ng (? 7?? 8?? 2,3 ?? 5,6 ),relatively stable(?A 3??B 5) , and irregular. (3) The ratio of ?B 4 /?A 2 increased gradually with the r at aging process. CONCLUSION: T he gradual phasic changes in relative quantity of crystallins reflect the aging status of rat crystalline.
