Model establishment and biological behaviour observation of mouse bla stocyst co-cultured with hepatocarcinoma cell lines with differently invasive and metasta tic potential in vitro
- VernacularTitle:小鼠囊胚与不同侵袭转移潜能肝癌细胞系共培养模型的建立及生物学行为观察
- Author:
Daqiang LI
;
Zhibiao WANG
;
Jin BAI
;
Jie ZHAO
;
Kai HU
;
Yuan WANG
;
Yonghong DU
- Publication Type:Journal Article
- Keywords:
Blastocyst;
Liver neoplasms;
Neoplasm invasivenes s;
Cell line
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore interaction and biological behaviou r changes of two kinds of cells-blastocysts and hepatocarcinoma cells in the same microenvi ronment. METHODS:The models of mouse blastocysts co-cultured wit h human hepatoca rcinoma cell lines were established, then biological behaviours and mutual effe c ts of the two kinds of cells in co-culture system were observed. RESULTS: Co mpared with control group, hepatocarcinoma cells with differently invasive and met astatic potential significantly enhanced the rates of blastocyst hatchment , at t achment and outgrowth(P0 05). The blastocyst ha tched and attached to hepatocarcinoma cells with differently invasive and metast atic p otential. Then, differential trophoblasts invaded hepatocarcinoma cells. The clear-cut interfaces were gradually formed between both sides. Hepatocarcinoma cells o n interface showed changes of growth direction and cell shapes and did not inv ade blastocysts. CONCLUSIONS: Hepatocarcinoma cells promoted bla stocyst develo pment. Blastocysts implanted and invaded hepatocarcinoma cells with differentl y i nvasive and metastatic potential in vitro, which indicate that blastocyst i mplan tation in vitro does not relate with the kinds and differential level of int erac tional cells and the low selectivity maybe relate with high adaptability of earl y life.
