Relations between adventitial cell proliferation, phenotype change and transforming growth factor ?1 expression after arterial injury in rabbits
- VernacularTitle:损伤家兔动脉后外膜成纤维细胞增殖、表型变化与TGF-?1表达的关系
- Author:
Ruqiong NIE
;
Jingfeng WANG
;
Wei WU
;
Qicai LIU
;
Yingsheng XUE
;
Xumin ZHANG
- Publication Type:Journal Article
- Keywords:
Arteries;
Fibroblasts;
Transforming growth f actor beta
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the relations between the proliferation and phenotype change of adventitial cells and the expression of T GF-?1 in adventitia after arterial injury. METHODS: After abdominal artery injury in rabbits, adventitial cel l proliferation and phenotype were identified by immunohistochemistry, transmissi on electron microscope and in situ Hybridization. RESULTS: Three days and 7 days after injury, the PCNA-positive ce lls were significantly increased. The previously weakly actin-positive adventiti al cells became strong-actin staining, the typical myofibroblast featurses of ad ventitial cells were detected by TEM 7 and 14 days after injury. Three days afte r vascular injury, there was a marked increase in the nu mber of cells that demonstrated the presence of TGF-?1 transcripts within the adventitia. Seven days after arterial injury, adventitial cells continued to ex press TGF-?1. on day 28, the number of TGF-?1-expressing cells was markedly r e duced in the adventitia. CONCLUSION: This study demonstrates that the proliferation and p henotype modulation of adventitial cells are associated with induction of TGF- ?1 expression in adventitia after arterial injury.
