Effects of p27kip1 gene on DNA and protein synthesis in esophageal carcinoma cells
- VernacularTitle:p27kip1基因对食管癌细胞DNA复制及蛋白质合成的影响
- Author:
Qiang TONG
;
Xiaohu WANG
;
Guojian XIE
;
Qingming WU
- Publication Type:Journal Article
- Keywords:
Genes, p27kip1;
Esophageal neoplasms;
DNA rep lication
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of p27kip1 ge ne on DNA replication and protein synthesis in esophageal carcinoma cells. METHODS: Recombinant adenovirus Ad-p27kip1 was constructed and transf ected into esophageal carcinoma cell EC-9706, and its effects on DNA replication , protein synthesis and the growth of esophageal carcinoma cells were explored b y means of cell growth count, -TdR, -Leucine incorporation meth od and flow cytometry. RESULTS: The growth of esophageal carcino ma cells was inhibited obviously. The radioactive intensity of -TdR and -Leucine in Ad-p27kip1 group decreased significantly than that in contr ol group after EC-9706 transfection (P
