The opening of neuronal mitochondria permeability transition pore affects respiratory function during thrombotic cerebral ischemia in tree shrews
- VernacularTitle:树鼩脑缺血时神经元线粒体渗透性转导孔开放对线粒体呼吸的影响
- Author:
Ying ZHANG
;
Shuqing LI
;
Yue LIU
- Publication Type:Journal Article
- Keywords:
Brain ischemia;
Mitochondrial respiration;
Permeability transition pore;
Ginkgolide B;
Tupaiidae;
Cyclosporine
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: The present study explored those changes in brain mitochondrial respiration in different times after thrombotic cerebral ischemia induced by photochemical reaction in primate's animal tree shrew and o bserved the effects of platelet-activating factor (PAF) receptor antagonist gin kgolide B (GB) and immunosuppressor cyclosporin A (CsA) on neuronal mitochondria l respiration and mitochondrial permeability transition pore(MPT) in twenty-four hours after occlusion and showed their neuronprotective mechanism and expatiate the relationship between mitochondrial respiration and MPT. METHODS: The focal thrombotic cerebral ischemia was formed by photochemistry-induce d technology in tree shrews. At 4,24,72 h after focal cerebral ischemia, the n euronal mitochondria were centrifuged. Clark oxygen electrode was used to measur e the changes in neuronal mitochondrial respiration.Pretreatment for experiment al animals with GB and CsA at 6h after occlusion, we centrifuged the mitochondri a and measured the changes in neuronal mitochondrial respiration at 24 h after o cclusion. In addition, experiments were performed in a flurometer by measuring CaCl 2 100 ?mol/L induced centrifuged mitochondrial swelling and GB or CsA were added at the same time. RESULTS: All of respiration state Ⅲ, RCR and P/O decreased after cerebral ischemia. There were significant difference s between every ischemic group and sham, especially at 24 h (P
