Effects of fluctuant high concentration of glucose on phosphoinositide3-kinase in skeletal muscle cells
- VernacularTitle:不同波动水平的高糖对大鼠骨骼肌细胞PI-3K表达的影响
- Author:
Bingbing LIU
;
Guoliang LIU
;
Xuehong ZHAO
- Publication Type:Journal Article
- Keywords:
Insulin resistance;
Phosphoinositude 3-kinase;
Muscle, skeletal;
Glucose
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effects of high glucose a t fluctuant concentrations on glucose transport activity and expression of phosp hoinositide 3-kinase (p85) in primary cultured skeletal muscle cells. ME T HODS: Rat skeletal muscle cells were cultured at fluctuant glucose conce ntrations (5, 25mmol/L) for 48h. Then the glucose uptake and the expression of p hosphoinositude 3-kinase were measured. RESULTS: The skeletal mu s cle cells treated with fluctuant high concentration glucose showed the impairmen t of the basal and insulin-induced increase in glucose uptake and significant de crease in p85 protein expression as well as p85 mRNA. Particularly, there was a significant decrease in p85 protein and mRNA expression in the high fluctuate l evel of glucose concentration. CONCLUSION: The exposure to fluct uant high concentration of glucose inhibits glucose uptake and induces insulin r esistance in skeletal muscle cells.
