Effects of diazoxide on ?-calpain activation, c-Fos and c-Jun expres sion in neonatal hypoxic-ischemic rat brain
- VernacularTitle:K_(ATP)通道开放剂对新生大鼠缺氧缺血后大脑?-calpain活化、c-Fos和c-Jun表达的影响
- Author:
Kewen JIANG
;
Quanxiang SHUI
;
Zhezhi XIA
;
Yin ZHANG
- Publication Type:Journal Article
- Keywords:
Potassium channels;
Calpain;
Diazoxide;
Hypox ia-ischemia, brain;
Proto-oncogene proteins c-fos;
Proto-oncogene proteins c-jun
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of diazoxide, an ATP-sensitive K + channel opener on the ?-calpain activation, c-Fos and c- Jun expression in neonatal hypoxic-ischemic rat brain. METHODS:The animal model of hypoxic-ischemic brain injury (HIBI) was made in the 7-day -old SD rats. Diazoxide was injected into the left lateral ventricle prior or po st hypoxic-ischemia (HI) insults. Western blot was applied to detect the integra ted density (ID) of the nuclear c-Fos and c-Jun at 4h, and the cleavage of cytos olic ?-calpain at 24 h after HI insults. RESULTS: Low c-Fos and c-Jun expressions from cortical and hippocampal samples were observed in the tw o diazoxide groups, and significant differences in their expressions were found by comparison with the HI controls (P
