Significance of NF-?B Activation Induced by LPS in Rat Kupffer Cells
- VernacularTitle:大肠杆菌脂多糖诱导大鼠Kupffer细胞NF-?B激活及其意义
- Author:
Lei WANG
;
Liping YU
;
Kefeng DOU
- Publication Type:Journal Article
- Keywords:
NF-?B;
LPS;
PDTC;
Kupffer cell
- From:
Journal of Chinese Physician
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of NF-?B activation induced by lipopolysaccharide(LPS) in rat Kupffer cells(KCs). Methods KCs were divided randomly into three groups. A group was control group. KCs were co-cultured with LPS in B group. KCs were co-cultured with LPS and PDTC in C group. NF-?B activity of KCs was determined with EMSA. Content of I-?B protein in KCs was detected with western blot. Expression levels of TNF-? mRNA, IL-6 mRNA of KCs were measured with RT-PCR. Results LPS induced NF-?B activation of KCs, decreased I-?B content of KCs, and increased expression levels of TNF-? mRNA and IL-6 mRNA markedly. PDTC could diminish the above effects of LPS on KCs. Conclusions NF-?B activation induced by LPS may induce cell injury by promoting expressions of TNF-? mRNA and IL-6 mRNA in KCs. PDTC could decrease mRNA expression of proinflammatory mediators by suppressing excessive NF-?B activation in KCs.
