A Competitive RT-PCR to Study Apolipoprotein E Gene Gxpression in Adipose Cells
- VernacularTitle:应用RT-PCR法分析人脂肪细胞载脂蛋白E基因表达
- Author:
Wei XIANG
;
Yanlin MA
;
Shengmiao FU
- Publication Type:Journal Article
- Keywords:
Apolipoprotein E;
Gene;
Reverse transcription;
PCR
- From:
Journal of Chinese Physician
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method to detect the expression of apolipoprotein E gene in human adipose cells. Methods Apolipoprotein E gene expression was determined by competitive reverse transcription-PCR in adipose cells of healthy children. Results Apolipoprotein E gene expressed in the adipose cells of healthy children. The mean expression quantity of apolipoprotein E mRNA in adipose cells was (0.41?0.12 ) in healthy children. Conclusions Competitive reverse transcription-PCR is a rapid, simple, sensitive and accurate method to analyze expression of apolipoprotein E gene.
